Cryopreservation (freezing) of semen


How we prepare insemination doses of canine sperm

  1. Semen is painlessly collected by digital manipulation, without any sedation.
  2. It does help if an in-season bitch is available. However, this is not always essential.
  3. After collection, semen quality is evaluated and the number of insemination doses is calculated.
  4. Two 0.5-ml plastic straws usually constitute one insemination dose.
  5. Quality of insemination doses is checked by evaluation of a frozen-thawed control straw.
  6. Insemination doses are stored in liquid nitrogen until applied or transported, according to the client’s wishes.
  7. Besides traditional returnable transport tanks, we offer one-way dry shipper Cryodrum for shipment of semen. The client does not pay for return of an empty container. Moreover, its weight is lower than that of common shipping containers with liquid nitrogen. However, there is a higher risk of its damage during transport!
  8. Frozen-thawed semen should be deposited directly into the uterus to increase the conception rate. Intrauterine insemination always requires a special approach: (1) using an endoscope or Scandinavian catheter for passing through the cervix to deposit semen into the uterus; (2) surgical insemination can also be done.
  9. The time of fertilization ability of frozen-thawed semen is much shorter in comparison with fresh semen.
  10. The insemination requires exact timing when the eggs are mature and ready for fertilization.
  11. The most reliable method for calculation of the optimum time for insemination is based on the blood progesterone test.

Transport and general conditions of exporting frozen canine semen

  • You should inquire about the conditions of semen export from the Czech Republic to any particular country at that country’s State Veterinary Administration.
  • If serological tests for brucelosis are required, the client should ask which tests are necessary in a particular country. The dog must be tested within a short time of semen collection.
  • We provide necessary documentation relating to semen transport.
  • The sizes of shipping containers:
    • CRYODRUM: diameter 32 cm, height 35 cm, weight of a full container 4.5 kg, dynamic endurance 4 days.
    • VOYAGER 2 l: 52 x 30 x 30 cm, weight of a full and empty container 8 kg and 6.5 kg, respectively; dynamic endurance 8 days.
    • VOYAGER 5 l: 67 x 40 x 40 cm, weight of a full and empty container 20 kg and 15.5 kg, respectively; dynamic endurance 23 days.
    • MVE SC4/3V: diameter 35 cm, height 59 cm weight of a full and empty container 14.5 kg and 10 kg, dynamic endurance 21 days.
  • Provided containers are Dry shippers, non hazardous, not restricted acc. to IATA special Provision A152.

Transport and general conditions of importing frozen canine semen to the Czech Republic

  • It is necessary to follow the requirements of the State Veterinary Administration (SVS) of the Czech Republic for the import of canine semen. (www.svscr.cz). In case the semen is imported from countries outside the EU, it is necessary to apply for import permission at the State Veterinary Administration at least 15 days in advance. We will be pleased to provide you with the information needed.